Nucleic Acids | | | |
Service | Platform | Cost per Sample | |
Nucleic Acid Isolation (RNA or DNA) | KingFisher Flex | $5 | |
Nucleic Acid Isolation (RNA or DNA) | Column | $10 | |
Nucleic Acid Isolation (RNA or DNA) | FFPE | $10 | |
QC Fragment Analysis (RNA, DNA, or pooled libraries) and Concentration Check | QIAxcel/Nanodrop | $5 | |
QC Fragment Analysis (RNA, DNA, pr pooled libraries) and Concentration Check | Agilent TapeStation 4200 | $10 | |
Fluorescent Nucleic Acid Quantitation (RNA, DNA, or pooled libraries) | Qubit | $2 | |
Total Nucleic Acid Service (Isolation, QC, and quantitation) | All of the above instruments | $10 | |
Covaris Sonication | M220 | free with training | |
|
Genotyping |
Service | Platform | Cost per Sample | |
Taqman (one-time charge for primers) | CFX96/CFX384 | $3 | |
PCR-based (one-time charge for primers) | QIAxcel | $3 | |
|
Real-time qPCR, Bio-Rad CFX-96/-384 |
Service | Platform | Cost per Sample | |
qRT-PCR (one-time charge for primers) | CFX96/CFX384 | $2 | |
|
Capillary Sequencing - SeqStudio | |
Service | Cost | | |
Full Service Sequencing (plasmid or PCR product) - per sample | Contact Us | | |
Full Service Sequencing (plasmid or PCR product) - 96-well plate | Contact Us | | |
Ready-to-Load Sequencing - 96-well plate | Contact Us | | |
Fragment Analysis (Ready-to-Load) - 96-well plate | Contact Us | | |
|
NGS-Based Pipelines |
Service | Nucleic Acid Input | Platform | Cost per Sample |
TruSeq Stranded RNA (Total) | Total RNA | Illumina NextSeq | $200 |
TruSeq Stranded RNA-mRNA | Total RNA | Illumina NextSeq | $150 |
NEXTFLEX Small RNA Sequencing Kit V4 | Small or Total RNA | Illumina NextSeq | $125 |
TruSeq DNA PCR-free (WGS) | Genomic DNA | Illumina NextSeq | $180 |
TruSeq DNA Methylation | Genomic DNA | Illumina NextSeq | $150 |
ScriptSeq Bacterial RNA | Total RNA | Illumina NextSeq | $125 |
Illumina DNA Prep (Small Genomes, Amplicons, Plasmids) | DNA, PCR products, Amplicons | Illumina NextSeq | $50 |
16S Metagenomics | Microbial DNA | Illumina NextSeq | $25 |
|
Automated Nucleic Acid/Library Preparation without Full Service |
Platform | Cost per Usage | | |
Kingfisher | $75 | | |
Zephyr G3 NGS Workstation | $250 | | |
|
Illumina iseq 100 Sequencing (up to 4 million reads) |
Service | Kit Cost | Per Run Charge | Total |
iSeq 100 i1 Reagent v2 (300 cycle) | $750 | $155 | $905 |
|
Illumina NextSeq 1000/2000 Sequencing (100 million - 1.1 billion reads) |
Service | Kit Cost | Per Run Charge | Total |
NextSeq 1000/2000 P1 Reagents (300 Cycles) | $1,400 | $350 | $1,750 |
NextSeq 1000/2000 P1 Reagents (600 Cycles) v3 | $2,100 | $350 | $2,450 |
NextSeq 1000/2000 P2 Reagents (100 cycles) v3 | $1,705 | $350 | $2,055 |
NextSeq 1000/2000 P2 Reagents (200 Cycles) v3 | $2,986 | $350 | $3,336 |
NextSeq 1000/2000 P2 Reagents (300 Cycles) v3 | $3,878 | $350 | $4,228 |
NextSeq 2000 P3 Reagents (50 Cycles) | $2,556 | $350 | $2,906 |
NextSeq 2000 P3 Reagents (100 Cycles) | $3,581 | $350 | $3,931 |
NextSeq 2000 P3 Reagents (200 Cycles) | $4,913 | $350 | $5,263 |
NextSeq 2000 P3 Reagents (300 Cycles) | $6,550 | $350 | $6,900 |
| | | |
Data Analysis |
Service | Cost | | |
Analysis of RNA sequencing data via Cloud BaseSpace (alignment and differential expression using DESeq2) - per sample | $20 | | |
Microbiome analysis | $5 | | |